Chemical Biology: Manipulating Macromolecules to Control Photoreactivity:
As our experimental efforts advance, we intend to employ macromolecular chaperones to steer the reaction through the branching space that determines products. Such development of photoenzymatic activity falls into a different class completely from evolution of novel enzymes using ground state reactivity. Rather than requiring wholesale changes to the ground state potentials to stabilize transition states, we only require slight steric effects to steer a reaction that already progresses under kinetic control. Using simple selection techniques, we hope to evolve novel photoenzymes to control the yields and products of reactions.
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Last Updated:
April 18, 2008
Engel Group
University of Chicago
GCIS E028
929 East 57th Street
Chicago, IL 60637
